2022
Lenárt, Sára; Lenárt, Peter; Knopfová, Lucia; Kotasová, Hana; Pelková, Vendula; Sedláková, Veronika; Vacek, Ondřej; Pokludová, Jana; Čan, Vladimír; Šmarda, Jan; Souček, Karel; Hampl, Aleš; Beneš, Petr
TACSTD2 upregulation is an early reaction to lung infection. Journal Article
In: Scientific reports, vol. 12, no. 1, pp. 9583, 2022, ISSN: 2045-2322, (Place: England).
Abstract | Links | BibTeX | Tags: *Antigens, *Cell Adhesion Molecules/metabolism, Animals, Epithelial Cells/metabolism, Lung/metabolism, Neoplasm/metabolism, Up-Regulation
@article{lenart_tacstd2_2022,
title = {TACSTD2 upregulation is an early reaction to lung infection.},
author = {Sára Lenárt and Peter Lenárt and Lucia Knopfová and Hana Kotasová and Vendula Pelková and Veronika Sedláková and Ondřej Vacek and Jana Pokludová and Vladimír Čan and Jan Šmarda and Karel Souček and Aleš Hampl and Petr Beneš},
doi = {10.1038/s41598-022-13637-9},
issn = {2045-2322},
year = {2022},
date = {2022-06-01},
journal = {Scientific reports},
volume = {12},
number = {1},
pages = {9583},
abstract = {TACSTD2 encodes a transmembrane glycoprotein Trop2 commonly overexpressed in carcinomas. While the Trop2 protein was discovered already in 1981 and first antibody-drug conjugate targeting Trop2 were recently approved for cancer therapy, the physiological role of Trop2 is still not fully understood. In this article, we show that TACSTD2/Trop2 expression is evolutionarily conserved in lungs of various vertebrates. By analysis of publicly available transcriptomic data we demonstrate that TACSTD2 level consistently increases in lungs infected with miscellaneous, but mainly viral pathogens. Single cell and subpopulation based transcriptomic data revealed that the major source of TACSTD2 transcript are lung epithelial cells and their progenitors and that TACSTD2 is induced directly in lung epithelial cells following infection. Increase in TACSTD2 expression may represent a mechanism to maintain/restore epithelial barrier function and contribute to regeneration process in infected/damaged lungs.},
note = {Place: England},
keywords = {*Antigens, *Cell Adhesion Molecules/metabolism, Animals, Epithelial Cells/metabolism, Lung/metabolism, Neoplasm/metabolism, Up-Regulation},
pubstate = {published},
tppubtype = {article}
}
TACSTD2 encodes a transmembrane glycoprotein Trop2 commonly overexpressed in carcinomas. While the Trop2 protein was discovered already in 1981 and first antibody-drug conjugate targeting Trop2 were recently approved for cancer therapy, the physiological role of Trop2 is still not fully understood. In this article, we show that TACSTD2/Trop2 expression is evolutionarily conserved in lungs of various vertebrates. By analysis of publicly available transcriptomic data we demonstrate that TACSTD2 level consistently increases in lungs infected with miscellaneous, but mainly viral pathogens. Single cell and subpopulation based transcriptomic data revealed that the major source of TACSTD2 transcript are lung epithelial cells and their progenitors and that TACSTD2 is induced directly in lung epithelial cells following infection. Increase in TACSTD2 expression may represent a mechanism to maintain/restore epithelial barrier function and contribute to regeneration process in infected/damaged lungs.
Drápela, Stanislav; Fedr, Radek; Vacek, Ondřej; Remšík, Ján; Souček, Karel
High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding. Journal Article
In: Methods in molecular biology (Clifton, N.J.), vol. 2543, pp. 99–111, 2022, ISSN: 1940-6029 1064-3745, (Place: United States).
Abstract | Links | BibTeX | Tags: *Antigens, *Research, Biomarkers/analysis, Cell surface phenotyping, Flow Cytometry, Fluorescent cell barcoding, Fluorescent Dyes, High-throughput screening, Multicolor flow cytometry, Surface
@article{drapela_high-throughput_2022,
title = {High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding.},
author = {Stanislav Drápela and Radek Fedr and Ondřej Vacek and Ján Remšík and Karel Souček},
doi = {10.1007/978-1-0716-2553-8_9},
issn = {1940-6029 1064-3745},
year = {2022},
date = {2022-01-01},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {2543},
pages = {99–111},
abstract = {Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.},
note = {Place: United States},
keywords = {*Antigens, *Research, Biomarkers/analysis, Cell surface phenotyping, Flow Cytometry, Fluorescent cell barcoding, Fluorescent Dyes, High-throughput screening, Multicolor flow cytometry, Surface},
pubstate = {published},
tppubtype = {article}
}
Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.