2020
Remšík, Ján; Pícková, Markéta; Vacek, Ondřej; Fedr, Radek; Binó, Lucia; Hampl, Aleš; Souček, Karel
TGF-β regulates Sca-1 expression and plasticity of pre-neoplastic mammary epithelial stem cells. Journal Article
In: Scientific reports, vol. 10, no. 1, pp. 11396, 2020, ISSN: 2045-2322, (Place: England).
Abstract | Links | BibTeX | Tags: Animal/pathology, Animals, Ataxin-1/*metabolism, Breast Neoplasms/genetics/*pathology, Cell Line, Cell Plasticity/genetics, Epithelial Cells/pathology, Epithelial-Mesenchymal Transition/genetics, ErbB-2/genetics, Experimental/genetics/*pathology, Female, Gene Expression Regulation, Humans, Mammary Glands, Mammary Neoplasms, Mice, Neoplastic, Neoplastic Stem Cells/*pathology, Receptor, Recombinant Proteins/genetics/metabolism, Signal Transduction/genetics, Transforming Growth Factor beta/genetics/*metabolism, Tumor/transplantation
@article{remsik_tgf-_2020,
title = {TGF-β regulates Sca-1 expression and plasticity of pre-neoplastic mammary epithelial stem cells.},
author = {Ján Remšík and Markéta Pícková and Ondřej Vacek and Radek Fedr and Lucia Binó and Aleš Hampl and Karel Souček},
doi = {10.1038/s41598-020-67827-4},
issn = {2045-2322},
year = {2020},
date = {2020-07-01},
journal = {Scientific reports},
volume = {10},
number = {1},
pages = {11396},
abstract = {The epithelial-mesenchymal plasticity, in tight association with stemness, contributes to the mammary gland homeostasis, evolution of early neoplastic lesions and cancer dissemination. Focused on cell surfaceome, we used mouse models of pre-neoplastic mammary epithelial and cancer stem cells to reveal the connection between cell surface markers and distinct cell phenotypes. We mechanistically dissected the TGF-β family-driven regulation of Sca-1, one of the most commonly used adult stem cell markers. We further provided evidence that TGF-β disrupts the lineage commitment and promotes the accumulation of tumor-initiating cells in pre-neoplastic cells.},
note = {Place: England},
keywords = {Animal/pathology, Animals, Ataxin-1/*metabolism, Breast Neoplasms/genetics/*pathology, Cell Line, Cell Plasticity/genetics, Epithelial Cells/pathology, Epithelial-Mesenchymal Transition/genetics, ErbB-2/genetics, Experimental/genetics/*pathology, Female, Gene Expression Regulation, Humans, Mammary Glands, Mammary Neoplasms, Mice, Neoplastic, Neoplastic Stem Cells/*pathology, Receptor, Recombinant Proteins/genetics/metabolism, Signal Transduction/genetics, Transforming Growth Factor beta/genetics/*metabolism, Tumor/transplantation},
pubstate = {published},
tppubtype = {article}
}
2018
Porokh, Volodymyr; Vaňhara, Petr; Bárta, Tomáš; Jurečková, Lucie; Bohačiaková, Dáša; Pospíšilová, Veronika; Mináriková, Daniela; Holubcová, Zuzana; Pelková, Vendula; Souček, Karel; Hampl, Aleš
Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells. Journal Article
In: Stem cells and development, vol. 27, no. 16, pp. 1077–1084, 2018, ISSN: 1557-8534 1547-3287, (Place: United States).
Abstract | Links | BibTeX | Tags: *Centrosome, Cell Differentiation/genetics, centrosomes, Cripto-1, culture adaptation, Embryonic Stem Cells, GPI-Linked Proteins/antagonists & inhibitors/*genetics, Human Embryonic Stem Cells/*cytology/metabolism, Humans, Intercellular Signaling Peptides and Proteins/*genetics, Mitosis/*genetics, multipolar mitoses, Neoplasm Proteins/antagonists & inhibitors/*genetics, Signal Transduction/genetics
@article{porokh_soluble_2018,
title = {Soluble Cripto-1 Induces Accumulation of Supernumerary Centrosomes and Formation of Aberrant Mitoses in Human Embryonic Stem Cells.},
author = {Volodymyr Porokh and Petr Vaňhara and Tomáš Bárta and Lucie Jurečková and Dáša Bohačiaková and Veronika Pospíšilová and Daniela Mináriková and Zuzana Holubcová and Vendula Pelková and Karel Souček and Aleš Hampl},
doi = {10.1089/scd.2018.0017},
issn = {1557-8534 1547-3287},
year = {2018},
date = {2018-08-01},
journal = {Stem cells and development},
volume = {27},
number = {16},
pages = {1077–1084},
abstract = {Chromosomal instability evoked by abnormalities in centrosome numbers has been traditionally considered as a hallmark of aberrant, typically cancerous or senescent cells. We have reported previously that pristine human embryonic stem cells (hESC) suffer from high frequency of supernumerary centrosomes and hence may be prone to undergo abnormal mitotic divisions. We have also unraveled that this phenomenon of multicentrosomal mitoses vanishes with prolonged time in culture and with initiation of differentiation, and it is strongly affected by the culture substratum. In this study, we report for the first time that Cripto-1 protein (teratocarcinoma-derived growth factor 1, epidermal growth factor-Cripto/FRL-1/Cryptic) produced by hESC represents a factor capable of inducing formation of supernumerary centrosomes in cultured hESC. Elimination of Cripto-1 signaling on the other hand restores the normal number of centrosomes in hESC. Linking the secretory phenotype of hESC to the centrosomal metabolism may help to develop better strategies for propagation of stable and safe bioindustrial and clinical grade cultures of hESC. From a broader point of view, it may lead to unravelling Cripto-1 as a micro-environmental factor contributing to adverse cell behaviors in vivo.},
note = {Place: United States},
keywords = {*Centrosome, Cell Differentiation/genetics, centrosomes, Cripto-1, culture adaptation, Embryonic Stem Cells, GPI-Linked Proteins/antagonists & inhibitors/*genetics, Human Embryonic Stem Cells/*cytology/metabolism, Humans, Intercellular Signaling Peptides and Proteins/*genetics, Mitosis/*genetics, multipolar mitoses, Neoplasm Proteins/antagonists & inhibitors/*genetics, Signal Transduction/genetics},
pubstate = {published},
tppubtype = {article}
}