Latest Publications

@article{svobodova_2378-tetrachlorodibenzo-p-dioxin_2019,

title = {2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) Disrupts Control of Cell Proliferation and Apoptosis in a Human Model of Adult Liver Progenitors.},

author = {Jana Svobodová and Jiřina Procházková and Markéta Kabátková and Martin Krkoška and Lenka Šmerdová and Helena Líbalová and Jan Topinka and Jiří Kléma and Alois Kozubík and Miroslav Machala and Jan Vondráček},

doi = {10.1093/toxsci/kfz202},

issn = {1096-0929},

year  = {2019},

date = {2019-12-01},

journal = {Toxicological sciences : an official journal of the Society of Toxicology},

volume = {172},

number = {2},

pages = {368–384},

abstract = {The aryl hydrocarbon receptor (AhR) activation has been shown to alter proliferation, apoptosis, or differentiation of adult rat liver progenitors. Here, we investigated the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-mediated AhR activation on a human model of bipotent liver progenitors, undifferentiated HepaRG cells. We used both intact undifferentiated HepaRG cells, and the cells with silenced Hippo pathway effectors, yes-associated protein 1 (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ), which play key role(s) in tissue-specific progenitor cell self-renewal and expansion, such as in liver, cardiac, or respiratory progenitors. TCDD induced cell proliferation in confluent undifferentiated HepaRG cells; however, following YAP, and, in particular, double YAP/TAZ knockdown, TCDD promoted induction of apoptosis. These results suggested that, unlike in mature hepatocytes, or hepatocyte-like cells, activation of the AhR may sensitize undifferentiated HepaRG cells to apoptotic stimuli. Induction of apoptosis in cells with silenced YAP/TAZ was associated with upregulation of death ligand TRAIL, and seemed to involve both extrinsic and mitochondrial apoptosis pathways. Global gene expression analysis further suggested that TCDD significantly altered expression of constituents and/or transcriptional targets of signaling pathways participating in control of expansion or differentiation of liver progenitors, including EGFR, Wnt/β-catenin, or tumor growth factor-β signaling pathways. TCDD significantly upregulated cytosolic proapoptotic protein BMF (Bcl-2 modifying factor) in HepaRG cells, which could be linked with an enhanced sensitivity of TCDD-treated cells to apoptosis. Our results suggest that, in addition to promotion of cell proliferation and alteration of signaling pathways controlling expansion of human adult liver progenitors, AhR ligands may also sensitize human liver progenitor cells to apoptosis.},

note = {Place: United States},

keywords = {*Models, Adaptor Proteins, Apoptosis, Apoptosis/*drug effects/genetics, Aryl hydrocarbon receptor, Aryl Hydrocarbon/metabolism, Biological, Cell Line, Cell Proliferation, Cell Proliferation/*drug effects/genetics, Gene Expression/drug effects, HepaRG cells, Hippo signaling, Humans, Liver/*drug effects/pathology, Polychlorinated Dibenzodioxins/*toxicity, Receptors, RNA, Signal Transducing/genetics, Signal Transduction, Small Interfering/genetics, Stem Cells/*drug effects/pathology, Trans-Activators/genetics, Transcription Factors/genetics, Transcriptional Coactivator with PDZ-Binding Motif Proteins, Transfection, YAP-Signaling Proteins},

pubstate = {published},

tppubtype = {article}

}

@article{kabatkova_inhibition_2015,

title = {Inhibition of β-catenin signalling promotes DNA damage elicited by benzo[a]pyrene in a model of human colon cancer cells via CYP1 deregulation.},

author = {Markéta Kabátková and Ondřej Zapletal and Zuzana Tylichová and Jiří Neča and Miroslav Machala and Alena Milcová and Jan Topinka and Alois Kozubík and Jan Vondráček},

doi = {10.1093/mutage/gev019},

issn = {1464-3804 0267-8357},

year  = {2015},

date = {2015-07-01},

journal = {Mutagenesis},

volume = {30},

number = {4},

pages = {565–576},

abstract = {Deregulation of Wnt/β-catenin signalling plays an important role in the pathogenesis of colorectal cancer. Interestingly, this pathway has been recently implicated in transcriptional control of cytochrome P450 (CYP) family 1 enzymes, which are responsible for bioactivation of a number of dietary carcinogens. In the present study, we investigated the impact of inhibition of Wnt/β-catenin pathway on metabolism and genotoxicity of benzo[a]pyrene (BaP), a highly mutagenic polycyclic aromatic hydrocarbon and an efficient ligand of the aryl hydrocarbon receptor, which is known as a primary regulator of CYP1 expression, in cellular models derived from colorectal tumours. We observed that a synthetic inhibitor of β-catenin, JW74, significantly increased formation of BaP-induced DNA adducts in both colorectal adenoma and carcinoma-derived cell lines. Using the short interfering RNA (siRNA) targeting β-catenin, we then found that β-catenin knockdown in HCT116 colon carcinoma cells significantly enhanced formation of covalent DNA adducts by BaP and histone H2AX phosphorylation, as detected by (32)P-postlabelling technique and immunocytochemistry, respectively, and it also induced expression of DNA damage response genes, such as CDKN1A or DDB2. The increased formation of DNA adducts formed by BaP upon β-catenin knockdown corresponded with enhanced production of major BaP metabolites, as well as with an increased expression/activity of CYP1 enzymes. Finally, using siRNA-mediated knockdown of CYP1A1, we confirmed that this enzyme plays a major role in formation of BaP-induced DNA adducts in HCT116 cells. Taken together, the present results indicated that the siRNA-mediated inhibition of β-catenin signalling, which is aberrantly activated in a majority of colorectal cancers, modulated genotoxicity of dietary carcinogen BaP in colon cell model in vitro, via a mechanism involving up-regulation of CYP1 expression and activity.},

note = {Place: England},

keywords = {*DNA Damage, Apoptosis, Aryl Hydrocarbon/genetics/metabolism, Benzo(a)pyrene/*adverse effects, beta Catenin/*antagonists & inhibitors/genetics/metabolism, Blotting, Carcinogens, Cell Proliferation, Colonic Neoplasms/drug therapy/*etiology/*pathology, Cultured, Cytochrome P-450 CYP1A1/antagonists & inhibitors/genetics/*metabolism, DNA Adducts/*adverse effects, Environmental/adverse effects, Enzymologic/*drug effects, Gene Expression Regulation, Humans, Immunoenzyme Techniques, Messenger/genetics, Neoplastic/*drug effects, Real-Time Polymerase Chain Reaction, Receptors, Reverse Transcriptase Polymerase Chain Reaction, RNA, Small Interfering/genetics, Tumor Cells, Western},

pubstate = {published},

tppubtype = {article}

}

@article{andrysik_aryl_2007,

title = {The aryl hydrocarbon receptor-dependent deregulation of cell cycle control induced by polycyclic aromatic hydrocarbons in rat liver epithelial cells.},

author = {Zdenek Andrysík and Jan Vondrácek and Miroslav Machala and Pavel Krcmár and Lenka Svihálková-Sindlerová and Anne Kranz and Carsten Weiss and Dagmar Faust and Alois Kozubík and Cornelia Dietrich},

doi = {10.1016/j.mrfmmm.2006.10.004},

issn = {0027-5107},

year  = {2007},

date = {2007-02-01},

journal = {Mutation research},

volume = {615},

number = {1-2},

pages = {87–97},

abstract = {Disruption of cell proliferation control by polycyclic aromatic hydrocarbons (PAHs) may contribute to their carcinogenicity. We investigated role of the aryl hydrocarbon receptor (AhR) in disruption of contact inhibition in rat liver epithelial WB-F344 'stem-like' cells, induced by the weakly mutagenic benz[a]anthracene (BaA), benzo[b]fluoranthene (BbF) and by the strongly mutagenic benzo[a]pyrene (BaP). There were significant differences between the effects of BaA and BbF, and those of the strongly genotoxic BaP. Both BaA and BbF increased percentage of cells entering S-phase and cell numbers, associated with an increased expression of Cyclin A and Cyclin A/cdk2 complex activity. Their effects were significantly reduced in cells expressing a dominant-negative AhR mutant (dnAhR). Roscovitine, a chemical inhibitor of cdk2, abolished the induction of cell proliferation by BbF. However, neither BaA nor BbF modulated expression of the principal cdk inhibitor involved in maintenance of contact inhibition, p27(Kip1), or pRb phosphorylation. The strongly mutagenic BaP induced apoptosis, a decrease in total cell numbers and significantly higher percentage of cells entering S-phase than either BaA or BbF. Given that BaP induced high levels of Cyclin A/cdk2 activity, downregulation of p27(Kip1) and hyperphosphorylation of pRb, the accumulation of cells in S-phase was probably due to cell proliferation, although S-phase arrest due to blocked replication forks can not be excluded. Both types of effects of BaP were significantly attenuated in dnAhR cells. Transfection of WB-F344 cells with siRNA targeted against AhR decreased induction of Cyclin A induced by BbF or BaP, further supporting the role of AhR in proliferative effects of PAHs. This suggest that activation of AhR plays a significant role both in disruption of contact inhibition by weakly mutagenic PAHs and in genotoxic effects of BaP possibly leading to enhanced cell proliferation. Thus, PAHs may increase proliferative rate and the likelihood of fixation of mutations.},

note = {Place: Netherlands},

keywords = {Animals, Apoptosis/drug effects, Aryl Hydrocarbon/antagonists & inhibitors/genetics/*metabolism, Base Sequence, Benz(a)Anthracenes/toxicity, Benzo(a)pyrene/toxicity, Cell Cycle Proteins/metabolism, Cell Cycle/*drug effects/*physiology, Cell Line, Cell Proliferation/drug effects, Cyclin A/metabolism, Cyclin-Dependent Kinase 2/metabolism, Cytochrome P-450 CYP1A1/genetics, Epithelial Cells/cytology/drug effects/metabolism, Fluorenes/toxicity, Gene Expression/drug effects, Hepatocytes/cytology/*drug effects/*metabolism, Messenger/genetics/metabolism, Multiprotein Complexes, Mutagens/toxicity, Mutation, Polycyclic Aromatic Hydrocarbons/*toxicity, Rats, Receptors, RNA, Small Interfering/genetics},

pubstate = {published},

tppubtype = {article}

}