2019
Kahounová, Zuzana; Slabáková, Eva; Binó, Lucia; Remšík, Ján; Fedr, Radek; Bouchal, Jan; Vrtěl, Radek; Jurečková, Lucie; Porokh, Volodymyr; Páralová, Darja; Hampl, Aleš; Souček, Karel
Generation of human iPSCs from fetal prostate fibroblasts HPrF. Journal Article
In: Stem cell research, vol. 35, pp. 101405, 2019, ISSN: 1876-7753 1873-5061, (Place: England).
Abstract | Links | BibTeX | Tags: *Cellular Reprogramming Techniques, *Fetus/cytology/embryology, *Fibroblasts/cytology/metabolism, *Induced Pluripotent Stem Cells/cytology/metabolism, *Prostate/cytology/embryology, Cellular Reprogramming, Humans, Kruppel-Like Factor 4, Male
@article{kahounova_generation_2019,
title = {Generation of human iPSCs from fetal prostate fibroblasts HPrF.},
author = {Zuzana Kahounová and Eva Slabáková and Lucia Binó and Ján Remšík and Radek Fedr and Jan Bouchal and Radek Vrtěl and Lucie Jurečková and Volodymyr Porokh and Darja Páralová and Aleš Hampl and Karel Souček},
doi = {10.1016/j.scr.2019.101405},
issn = {1876-7753 1873-5061},
year = {2019},
date = {2019-03-01},
journal = {Stem cell research},
volume = {35},
pages = {101405},
abstract = {Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.},
note = {Place: England},
keywords = {*Cellular Reprogramming Techniques, *Fetus/cytology/embryology, *Fibroblasts/cytology/metabolism, *Induced Pluripotent Stem Cells/cytology/metabolism, *Prostate/cytology/embryology, Cellular Reprogramming, Humans, Kruppel-Like Factor 4, Male},
pubstate = {published},
tppubtype = {article}
}
Human induced pluripotent stem cell line was generated from commercially available primary human prostate fibroblasts HPrF derived from a fetus, aged 18-24 weeks of gestation. The fibroblast cell line was reprogrammed with Yamanaka factors (OCT4, SOX2, c-MYC, KLF4) using CytoTune™-iPS 2.0 Sendai Reprogramming Kit. Pluripotency of the derived transgene-free iPS cell line was confirmed both in vitro by detecting the expression of factors of pluripotency on a single-cell level, and in vivo using teratoma formation assay. This iPS cell line will be a useful tool for studying both normal prostate development and prostate cancer disease.