2019
Šimek, Matěj; Hermannová, Martina; Šmejkalová, Daniela; Foglová, Tereza; Souček, Karel; Binó, Lucia; Velebný, Vladimír
LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin. Journal Article
In: Carbohydrate polymers, vol. 209, pp. 181–189, 2019, ISSN: 1879-1344 0144-8617, (Place: England).
Abstract | Links | BibTeX | Tags: *Micelles, Animals, Biodistribution, Chromatography, Doxorubicin, Doxorubicin/*chemistry/pharmacokinetics, Drug Carriers/*chemistry, Drug Liberation, Female, Hyaluronan, Hyaluronic Acid/*chemistry, Liquid, Mice, Molecular Weight, Pharmacokinetics, Polymeric micelles, Tandem Mass Spectrometry, Tissue Distribution
@article{simek_lc-msms_2019,
title = {LC-MS/MS study of in vivo fate of hyaluronan polymeric micelles carrying doxorubicin.},
author = {Matěj Šimek and Martina Hermannová and Daniela Šmejkalová and Tereza Foglová and Karel Souček and Lucia Binó and Vladimír Velebný},
doi = {10.1016/j.carbpol.2018.12.104},
issn = {1879-1344 0144-8617},
year = {2019},
date = {2019-04-01},
journal = {Carbohydrate polymers},
volume = {209},
pages = {181–189},
abstract = {A better understanding of in vivo behavior of nanocarriers is necessary for further improvement in their development. Here we present a novel approach, where both the matrix and the drug can be analyzed by LCMS/MS after one sample handling. The developed method was applied for the comparison of pharmacokinetic profile of free and encapsulated doxorubicin (DOX) in oleyl hyaluronan (HA-C18:1) polymeric micelles. The results indicated that nanocarriers were rapidly dissociated upon in vivo administration. Despite this fact, the administration of encapsulated DOX led to its longer circulation time and enhanced tumor targeting. This effect was not observed injecting blank HA-C18:1 micelles followed by unencapsulated DOX. Biodistribution studies and molecular weight estimation of the carrier matrix indicated relatively high stability of HA-C18:1 ester bond in bloodstream and complete elimination of the derivative within 72 h. The proposed methodology provides a novel strategy to elucidate the pharmacokinetic behavior of polysaccharide-based drug delivery systems.},
note = {Place: England},
keywords = {*Micelles, Animals, Biodistribution, Chromatography, Doxorubicin, Doxorubicin/*chemistry/pharmacokinetics, Drug Carriers/*chemistry, Drug Liberation, Female, Hyaluronan, Hyaluronic Acid/*chemistry, Liquid, Mice, Molecular Weight, Pharmacokinetics, Polymeric micelles, Tandem Mass Spectrometry, Tissue Distribution},
pubstate = {published},
tppubtype = {article}
}
A better understanding of in vivo behavior of nanocarriers is necessary for further improvement in their development. Here we present a novel approach, where both the matrix and the drug can be analyzed by LCMS/MS after one sample handling. The developed method was applied for the comparison of pharmacokinetic profile of free and encapsulated doxorubicin (DOX) in oleyl hyaluronan (HA-C18:1) polymeric micelles. The results indicated that nanocarriers were rapidly dissociated upon in vivo administration. Despite this fact, the administration of encapsulated DOX led to its longer circulation time and enhanced tumor targeting. This effect was not observed injecting blank HA-C18:1 micelles followed by unencapsulated DOX. Biodistribution studies and molecular weight estimation of the carrier matrix indicated relatively high stability of HA-C18:1 ester bond in bloodstream and complete elimination of the derivative within 72 h. The proposed methodology provides a novel strategy to elucidate the pharmacokinetic behavior of polysaccharide-based drug delivery systems.
2018
Achbergerová, Eva; Šmejkalová, Daniela; Huerta-Angeles, Gloria; Souček, Karel; Hermannová, Martina; Vágnerová, Hana; Vícha, Robert; Velebný, Vladimír
In vivo monitoring of tumor distribution of hyaluronan polymeric micelles labeled or loaded with near-infrared fluorescence dye. Journal Article
In: Carbohydrate polymers, vol. 198, pp. 339–347, 2018, ISSN: 1879-1344 0144-8617, (Place: England).
Abstract | Links | BibTeX | Tags: Hyaluronan, NIR fluorescence imaging, Polymeric micelles, Tumor detection
@article{achbergerova_vivo_2018,
title = {In vivo monitoring of tumor distribution of hyaluronan polymeric micelles labeled or loaded with near-infrared fluorescence dye.},
author = {Eva Achbergerová and Daniela Šmejkalová and Gloria Huerta-Angeles and Karel Souček and Martina Hermannová and Hana Vágnerová and Robert Vícha and Vladimír Velebný},
doi = {10.1016/j.carbpol.2018.06.082},
issn = {1879-1344 0144-8617},
year = {2018},
date = {2018-10-01},
journal = {Carbohydrate polymers},
volume = {198},
pages = {339–347},
abstract = {Development of delivery systems which allow real-time visual inspection of tumors is critical for effective therapy. Near-infrared (NIR) fluorophores have a great potential for such an application. To overcome NIR dyes short blood circulation time and increase tumor accumulation, a NIR dye, cypate, was associated with oleyl hyaluronan, which can self-assemble into polymeric aggregates. The cypate association with oleyl hyaluronan was performed either by a covalent linkage, or physical entrapment. The two systems were compared for tumor targeting and contrast enhancement using BALB/c mice bearing 4T1 breast cancer tumors. Independently on the way of cypate association, it took more than 24 h from intravenous administration to detect NIR signal in tumors and the tumors were clearly visualized for 2 following weeks without substrate reinjection. Covalently linked cypate generated 2-3 fold stronger fluorescence signal than physically loaded cypate. This study demonstrates the potential of HA matrix to be used as carrier of contrast agents for non-invasive long-term tumor visualization.},
note = {Place: England},
keywords = {Hyaluronan, NIR fluorescence imaging, Polymeric micelles, Tumor detection},
pubstate = {published},
tppubtype = {article}
}
Development of delivery systems which allow real-time visual inspection of tumors is critical for effective therapy. Near-infrared (NIR) fluorophores have a great potential for such an application. To overcome NIR dyes short blood circulation time and increase tumor accumulation, a NIR dye, cypate, was associated with oleyl hyaluronan, which can self-assemble into polymeric aggregates. The cypate association with oleyl hyaluronan was performed either by a covalent linkage, or physical entrapment. The two systems were compared for tumor targeting and contrast enhancement using BALB/c mice bearing 4T1 breast cancer tumors. Independently on the way of cypate association, it took more than 24 h from intravenous administration to detect NIR signal in tumors and the tumors were clearly visualized for 2 following weeks without substrate reinjection. Covalently linked cypate generated 2-3 fold stronger fluorescence signal than physically loaded cypate. This study demonstrates the potential of HA matrix to be used as carrier of contrast agents for non-invasive long-term tumor visualization.