2019
Tylichová, Zuzana; Neča, Jiří; Topinka, Jan; Milcová, Alena; Hofmanová, Jiřina; Kozubík, Alois; Machala, Miroslav; Vondráček, Jan
n-3 Polyunsaturated fatty acids alter benzo[a]pyrene metabolism and genotoxicity in human colon epithelial cell models. Journal Article
In: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, vol. 124, pp. 374–384, 2019, ISSN: 1873-6351 0278-6915, (Place: England).
Abstract | Links | BibTeX | Tags: Anticarcinogenic Agents/*pharmacology, Benzo(a)pyrene/adverse effects/*metabolism, Cell Line, Colon cancer, Cytochrome P450 Family 1/metabolism, DNA Adducts/metabolism, DNA Damage, DNA Damage/drug effects, Docosahexaenoic acid, Docosahexaenoic Acids/*pharmacology, Eicosapentaenoic acid, Eicosapentaenoic Acid/*pharmacology, Epithelial Cells/*drug effects, Histones/metabolism, Humans, Mutagens/adverse effects/*metabolism, Polycyclic aromatic hydrocarbon, S Phase Cell Cycle Checkpoints/drug effects, Tumor
@article{tylichova_n-3_2019,
title = {n-3 Polyunsaturated fatty acids alter benzo[a]pyrene metabolism and genotoxicity in human colon epithelial cell models.},
author = {Zuzana Tylichová and Jiří Neča and Jan Topinka and Alena Milcová and Jiřina Hofmanová and Alois Kozubík and Miroslav Machala and Jan Vondráček},
doi = {10.1016/j.fct.2018.12.021},
issn = {1873-6351 0278-6915},
year = {2019},
date = {2019-02-01},
journal = {Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association},
volume = {124},
pages = {374–384},
abstract = {Dietary carcinogens, such as benzo[a]pyrene (BaP), are suspected to contribute to colorectal cancer development. n-3 Polyunsaturated fatty acids (PUFAs) decrease colorectal cancer risk in individuals consuming diets rich in PUFAs. Here, we investigated the impact of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid on metabolism and genotoxicity of BaP in human cell models derived from the colon: HT-29 and HCT-116 cell lines. Both PUFAs reduced levels of excreted BaP metabolites, in particular BaP-tetrols and hydroxylated BaP metabolites, as well as formation of DNA adducts in HT-29 and HCT-116 cells. However, EPA appeared to be a more potent inhibitor of formation of some intracellular BaP metabolites, including BaP-7,8-dihydrodiol. EPA also reduced phosphorylation of histone H2AX (Ser139) in HT-29 cells, which indicated that it may reduce further forms of DNA damage, including DNA double strand breaks. Both PUFAs inhibited induction of CYP1 activity in colon cells determined as 7-ethoxyresorufin-O-deethylase (EROD); this was at least partly linked with inhibition of induction of CYP1A1, 1A2 and 1B1 mRNAs. The downregulation and/or inhibition of CYP1 enzymes by PUFAs could thus alter metabolism and reduce genotoxicity of BaP in human colon cells, which might contribute to known chemopreventive effects of PUFAs in colon epithelium.},
note = {Place: England},
keywords = {Anticarcinogenic Agents/*pharmacology, Benzo(a)pyrene/adverse effects/*metabolism, Cell Line, Colon cancer, Cytochrome P450 Family 1/metabolism, DNA Adducts/metabolism, DNA Damage, DNA Damage/drug effects, Docosahexaenoic acid, Docosahexaenoic Acids/*pharmacology, Eicosapentaenoic acid, Eicosapentaenoic Acid/*pharmacology, Epithelial Cells/*drug effects, Histones/metabolism, Humans, Mutagens/adverse effects/*metabolism, Polycyclic aromatic hydrocarbon, S Phase Cell Cycle Checkpoints/drug effects, Tumor},
pubstate = {published},
tppubtype = {article}
}