2018
Achbergerová, Eva; Šmejkalová, Daniela; Huerta-Angeles, Gloria; Souček, Karel; Hermannová, Martina; Vágnerová, Hana; Vícha, Robert; Velebný, Vladimír
In vivo monitoring of tumor distribution of hyaluronan polymeric micelles labeled or loaded with near-infrared fluorescence dye. Journal Article
In: Carbohydrate polymers, vol. 198, pp. 339–347, 2018, ISSN: 1879-1344 0144-8617, (Place: England).
Abstract | Links | BibTeX | Tags: Hyaluronan, NIR fluorescence imaging, Polymeric micelles, Tumor detection
@article{achbergerova_vivo_2018,
title = {In vivo monitoring of tumor distribution of hyaluronan polymeric micelles labeled or loaded with near-infrared fluorescence dye.},
author = {Eva Achbergerová and Daniela Šmejkalová and Gloria Huerta-Angeles and Karel Souček and Martina Hermannová and Hana Vágnerová and Robert Vícha and Vladimír Velebný},
doi = {10.1016/j.carbpol.2018.06.082},
issn = {1879-1344 0144-8617},
year = {2018},
date = {2018-10-01},
journal = {Carbohydrate polymers},
volume = {198},
pages = {339–347},
abstract = {Development of delivery systems which allow real-time visual inspection of tumors is critical for effective therapy. Near-infrared (NIR) fluorophores have a great potential for such an application. To overcome NIR dyes short blood circulation time and increase tumor accumulation, a NIR dye, cypate, was associated with oleyl hyaluronan, which can self-assemble into polymeric aggregates. The cypate association with oleyl hyaluronan was performed either by a covalent linkage, or physical entrapment. The two systems were compared for tumor targeting and contrast enhancement using BALB/c mice bearing 4T1 breast cancer tumors. Independently on the way of cypate association, it took more than 24 h from intravenous administration to detect NIR signal in tumors and the tumors were clearly visualized for 2 following weeks without substrate reinjection. Covalently linked cypate generated 2-3 fold stronger fluorescence signal than physically loaded cypate. This study demonstrates the potential of HA matrix to be used as carrier of contrast agents for non-invasive long-term tumor visualization.},
note = {Place: England},
keywords = {Hyaluronan, NIR fluorescence imaging, Polymeric micelles, Tumor detection},
pubstate = {published},
tppubtype = {article}
}
Development of delivery systems which allow real-time visual inspection of tumors is critical for effective therapy. Near-infrared (NIR) fluorophores have a great potential for such an application. To overcome NIR dyes short blood circulation time and increase tumor accumulation, a NIR dye, cypate, was associated with oleyl hyaluronan, which can self-assemble into polymeric aggregates. The cypate association with oleyl hyaluronan was performed either by a covalent linkage, or physical entrapment. The two systems were compared for tumor targeting and contrast enhancement using BALB/c mice bearing 4T1 breast cancer tumors. Independently on the way of cypate association, it took more than 24 h from intravenous administration to detect NIR signal in tumors and the tumors were clearly visualized for 2 following weeks without substrate reinjection. Covalently linked cypate generated 2-3 fold stronger fluorescence signal than physically loaded cypate. This study demonstrates the potential of HA matrix to be used as carrier of contrast agents for non-invasive long-term tumor visualization.