2022
Drápela, Stanislav; Fedr, Radek; Vacek, Ondřej; Remšík, Ján; Souček, Karel
High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding. Journal Article
In: Methods in molecular biology (Clifton, N.J.), vol. 2543, pp. 99–111, 2022, ISSN: 1940-6029 1064-3745, (Place: United States).
Abstract | Links | BibTeX | Tags: *Antigens, *Research, Biomarkers/analysis, Cell surface phenotyping, Flow Cytometry, Fluorescent cell barcoding, Fluorescent Dyes, High-throughput screening, Multicolor flow cytometry, Surface
@article{drapela_high-throughput_2022,
title = {High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding.},
author = {Stanislav Drápela and Radek Fedr and Ondřej Vacek and Ján Remšík and Karel Souček},
doi = {10.1007/978-1-0716-2553-8_9},
issn = {1940-6029 1064-3745},
year = {2022},
date = {2022-01-01},
journal = {Methods in molecular biology (Clifton, N.J.)},
volume = {2543},
pages = {99–111},
abstract = {Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.},
note = {Place: United States},
keywords = {*Antigens, *Research, Biomarkers/analysis, Cell surface phenotyping, Flow Cytometry, Fluorescent cell barcoding, Fluorescent Dyes, High-throughput screening, Multicolor flow cytometry, Surface},
pubstate = {published},
tppubtype = {article}
}
Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology.