Latest Publications

@article{trilecova_toxic_2011,

title = {Toxic effects of methylated benzo[a]pyrenes in rat liver stem-like cells.},

author = {Lenka Trilecová and Simona Krčková and Soňa Marvanová and Kateřina Pĕnčíková and Pavel Krčmář and Jiří Neča and Petra Hulinková and Lenka Pálková and Miroslav Ciganek and Alena Milcová and Jan Topinka and Jan Vondráček and Miroslav Machala},

doi = {10.1021/tx200049x},

issn = {1520-5010 0893-228X},

year  = {2011},

date = {2011-06-01},

journal = {Chemical research in toxicology},

volume = {24},

number = {6},

pages = {866–876},

abstract = {The methylated benzo[a]pyrenes (MeBaPs) are present at significant levels in the environment, especially in the sediments contaminated by petrogenic PAHs. However, the existing data on their toxic effects in vitro and/or in vivo are still largely incomplete. Transcription factor AhR plays a key role in the metabolic activation of PAHs to genotoxic metabolites, but the AhR activation may also contribute to the tumor promoting effects of PAHs. In this study, the AhR-mediated activity of five selected MeBaP isomers was estimated in the DR-CALUX reporter gene assay performed in rat hepatoma cells. Detection of other effects, including induction of CYP1A1, CYP1B1, and AKR1C9 mRNAs, DNA adduct formation, production of reactive oxygen species, oxidation of deoxyguanosine, and cell cycle modulation and apoptosis, was performed in the rat liver epithelial WB-F344 cell line, a model of liver progenitor cells. We identified 1-MeBaP as the most potent inducer of AhR activation, stable DNA adduct formation, checkpoint kinase 1 and p53 phosphorylation, and apoptosis. These effects suggest that 1-MeBaP is a potent genotoxin eliciting a typical sequence of events ascribed to carcinogenic PAHs: induction of CYP1 enzymes, formation of high levels of DNA adducts, activation of DNA damage responses (including p53 phosphorylation), and cell death. In contrast, 10-MeBaP, representing BaP isomers substituted with the methyl group in the angular ring, elicited only low levels DNA adduct formation and apoptosis. Other MeBaPs under study also elicited strong apoptotic responses associated with DNA adduct formation as the prevalent mode of toxic action of these compounds in liver cells. MeBaPs induced a weak production of ROS, which did not lead to significant oxidative DNA damage. Importantly, 1-MeBaP and 3-MeBaP were found to be potent AhR agonists, one order of magnitude more potent than BaP, thus suggesting that the AhR-dependent modulations of gene expression, deregulation of cell survival mechanisms, and further nongenotoxic effects associated with AhR activation may further contribute to their tumor promotion and carcinogenicity.},

note = {Place: United States},

keywords = {Animals, Apoptosis/drug effects, Aryl Hydrocarbon/*metabolism, Benzo(a)pyrene/*chemistry/*toxicity, Cell Cycle/drug effects, Cell Line, Checkpoint Kinase 1, DNA Adducts/metabolism, Epithelial Cells/drug effects/metabolism, Gene Expression Regulation/drug effects, Liver/*cytology, Methylation, Mutagens/*chemistry/*toxicity, Oxidative Stress/drug effects, Protein Kinases/metabolism, Rats, Receptors, Stem Cells/drug effects/metabolism, Tumor, Tumor Suppressor Protein p53/metabolism},

pubstate = {published},

tppubtype = {article}

}

@article{valovicova_differences_2009,

title = {Differences in DNA damage and repair produced by systemic, hepatocarcinogenic and sarcomagenic dibenzocarbazole derivatives in a model of rat liver progenitor cells.},

author = {Zuzana Valovicová and Sona Marvanová and Monika Mészárosová and Annamária Srancíková and Lenka Trilecová and Alena Milcová and Helena Líbalová and Jan Vondrácek and Miroslav Machala and Jan Topinka and Alena Gábelová},

doi = {10.1016/j.mrfmmm.2009.02.014},

issn = {0027-5107},

year  = {2009},

date = {2009-06-01},

journal = {Mutation research},

volume = {665},

number = {1-2},

pages = {51–60},

abstract = {Liver progenitor (oval) cells are a potential target cell population for hepatocarcinogens. Our recent study showed that the liver carcinogens 7H-dibenzo[c,g]carbazole (DBC) and 5,9-dimethyldibenzo[c,g]carbazole (DiMeDBC), but not the sarcomagen N-methyldibenzo[c,g]carbazole (N-MeDBC), induced several cellular events associated with tumor promotion in WB-F344 cells, an in vitro model of liver oval cells [J. Vondracek, L. Svihalkova-Sindlerova, K. Pencikova, P. Krcmar, Z. Andrysik, K. Chramostova, S. Marvanova, Z. Valovicova, A. Kozubik, A. Gabelova, M. Machala, 7H-Dibenzo[c,g]carbazole and 5,9-dimethyldibenzo[c,g]carbazole exert multiple toxic events contributing to tumor promotion in rat liver epithelial 'stem-like' cells, Mutat. Res. Fundam. Mol. Mech. Mutagen. 596 (2006) 43-56]. In this study, we focused on the genotoxic effects generated by these dibenzocarbazoles in WB-F344 cells to better understand the cellular and molecular mechanisms involved in hepatocarcinogenesis. Lower IC(50) values determined for DBC and DiMeDBC, as compared with N-MeDBC, indicated a higher sensitivity of WB-F344 cells towards hepatocarcinogens. Accordingly, DBC produced a dose-dependent DNA-adduct formation resulting in substantial inhibition of DNA replication and transcription. In contrast, DNA-adduct number detected in DiMeDBC-exposed cells was almost negligible, whereas N-MeDBC produced a low level of DNA adducts. Although all dibenzocarbazoles significantly increased the level of strand breaks (p<0.05) and micronuclei (p<0.001) after 2-h treatment, differences in the kinetics of strand break rejoining were found. The strand break level in DiMeDBC- and N-MeDBC-exposed cells returned to near the background level within 24h after treatment, whereas a relatively high DNA damage level was detected in DBC-treated cells up to 48h after exposure. Additional breaks detected after incubation of DiMeDBC-exposed WB-F344 cells with a repair-specific endonuclease, along with a nearly 3-fold higher level of reactive oxygen species found in these cells as compared with control, suggest a possible role of oxidative stress in DiMeDBC genotoxicity. We demonstrated qualitative differences in the DNA damage profiles produced by hepatocarcinogens DBC and DiMeDBC in WB-F344 cells. Different lesions may trigger distinct cellular pathways involved in hepatocarcinogenesis. The low amount of DNA damage, together with an efficient repair, may explain the lack of hepatocarcinogenicity of N-MeDBC.},

note = {Place: Netherlands},

keywords = {*DNA Damage, *DNA Repair, Animals, Biological, Carbazoles/*toxicity, Carcinogens/*toxicity, Cell Line, DNA Adducts/metabolism, Experimental/chemically induced, Histones/metabolism, Kinetics, Liver Neoplasms, Liver/cytology/*drug effects/*metabolism, Models, Mutagens/toxicity, Oxidative Stress/drug effects, Rats, Sarcoma, Stem Cells/cytology/*drug effects/*metabolism},

pubstate = {published},

tppubtype = {article}

}