2003
Nemajerová, Alice; Smarda, Jan; Jurdic, Pierre; Kubala, Lukás; Soucek, Karel; Smardová, Jana
Trichostatin A suppresses transformation by the v-myb oncogene in BM2 cells. Journal Article
In: Journal of hematotherapy & stem cell research, vol. 12, no. 2, pp. 225–235, 2003, ISSN: 1525-8165, (Place: United States).
Abstract | Links | BibTeX | Tags: Acetylation, Animals, Cell Cycle, Cell Differentiation, Cell Line, Cell Transformation, Chickens, Chromatin Assembly and Disassembly/physiology, Genes, Histone Deacetylases/drug effects, Histones/metabolism/physiology, Hydroxamic Acids/*pharmacology, Macrophages/cytology, myb/drug effects/*physiology, Transformed, Viral/*drug effects
@article{nemajerova_trichostatin_2003,
title = {Trichostatin A suppresses transformation by the v-myb oncogene in BM2 cells.},
author = {Alice Nemajerová and Jan Smarda and Pierre Jurdic and Lukás Kubala and Karel Soucek and Jana Smardová},
doi = {10.1089/152581603321628368},
issn = {1525-8165},
year = {2003},
date = {2003-04-01},
journal = {Journal of hematotherapy & stem cell research},
volume = {12},
number = {2},
pages = {225–235},
abstract = {BM2 cells are chicken monoblasts transformed by the v-myb oncogene of avian myeloblastosis virus. The constitutively high v-myb expression interferes with the terminal differentiation of BM2 cells, but these cells can be induced to differentiate into macrophage-like cells by phorbol esters. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to the regulation and pathology of hematopoiesis. In the present study, we examined the contribution of elevated histone acetylation to the differentiation of BM2 cells. Inhibition of the activity of endogenous histone deacetylases by trichostatin A (TSA) resulted in histone hyperacetylation causing cell cycle arrest and differentiation of BM2 cells into macrophage polykaryons. TSA did not affect the level of v-Myb protein in BM2 cells, but it downregulated its transcription activation capability. This suggests that chromatin remodeling can be significantly engaged in regulation of proliferation and differentiation of leukemic cells.},
note = {Place: United States},
keywords = {Acetylation, Animals, Cell Cycle, Cell Differentiation, Cell Line, Cell Transformation, Chickens, Chromatin Assembly and Disassembly/physiology, Genes, Histone Deacetylases/drug effects, Histones/metabolism/physiology, Hydroxamic Acids/*pharmacology, Macrophages/cytology, myb/drug effects/*physiology, Transformed, Viral/*drug effects},
pubstate = {published},
tppubtype = {article}
}
BM2 cells are chicken monoblasts transformed by the v-myb oncogene of avian myeloblastosis virus. The constitutively high v-myb expression interferes with the terminal differentiation of BM2 cells, but these cells can be induced to differentiate into macrophage-like cells by phorbol esters. Histone acetylation plays an important role in regulation of transcription and is particularly relevant to the regulation and pathology of hematopoiesis. In the present study, we examined the contribution of elevated histone acetylation to the differentiation of BM2 cells. Inhibition of the activity of endogenous histone deacetylases by trichostatin A (TSA) resulted in histone hyperacetylation causing cell cycle arrest and differentiation of BM2 cells into macrophage polykaryons. TSA did not affect the level of v-Myb protein in BM2 cells, but it downregulated its transcription activation capability. This suggests that chromatin remodeling can be significantly engaged in regulation of proliferation and differentiation of leukemic cells.